We propose a novel model consisting of six ordinary differential equations, wired by a feedback regulatory network to investigate the dynamics of hole recovery after HPP. The flow cytometry result with two fluorescent molecules at different time points after pressurization supports the existence of a recovery process. We quantify this damage by estimating the radius of holes created on the membrane after pressure exposure of 400 lasting for 8 and 20. Based on our observation using electron microscopy and flow cytometry techniques, membrane of Listeria monocytogenes is one of the main structures in this microorganism which is damaged by pressurization. However, a major concern in this field is the ability of some pathogens such as Listeria monocytogenes to recover pressure-induced damages after HPP even during cold temperature storage. High pressure processing (HPP) in the food industry is considered as an alternative to conventional thermal treatments for inactivation of pathogenic bacteria.
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